Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:200
Immunofluorescence (Immunocytochemistry): 1:200 - 1:800
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
GOLGA4/GOLGIN-245 (F2V4L) Rabbit Monoclonal Antibody recognizes endogenous levels of total GOLGA4/GOLGIN-245 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human GOLGA4/GOLGIN-245 protein.
Background
The Golgi-associated protein golgin A1 (GOLGA1, golgin-97) was first isolated as a Golgi complex autoantigen associated with the autoimmune disorder Sjogren's syndrome (1). The golgin-97 protein contains a carboxy-terminal GRIP domain and is a commonly used trans-Golgi network (TGN) marker. All four known mammalian GRIP domain-containing proteins (golgin-97, golgin-245, GCC88, and GCC185) are found in the TGN, share extensive alpha-helical structure, and form homodimers (2). While all four golgin proteins localize to the TGN, they exhibit different membrane-binding abilities and are found in distinct TGN regions (3). Golgin-97 and golgin-245 are targeted to the TGN through an interaction between their GRIP domains and the Arl1 protein switch II region (4). Overexpression studies and siRNA assays with GRIP domain-containing proteins suggest that these proteins help to maintain TGN integrity and function by controlling localization of TGN resident proteins (5). By using a Shiga toxin B fragment (STxB)-based transport assay and an E-cadherin transport model system, golgin-97 and its effector Arl1-GTP were shown to play a role in trans-Golgi endosomal trafficking (6,7). Research studies also suggest that golgin-97 may play a role in poxvirus morphogenesis and maturation (8,9).
Alternate Names
256 kDa golgin; 72.1 protein; centrosome-related protein F46; CRPF46; GCP2; GOGA4; GOLG; GOLGA4; golgi autoantigen, golgin subfamily a, 4; golgin A4; Golgin subfamily A member 4; golgin-240; Golgin-245; MU-RMS-40.18; p230; Protein 72.1; Trans-Golgi p230
