Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Immunohistochemistry (Paraffin): 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 85470 .
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin)
Specificity / Sensitivity
HOXB13 (D7N8O) Rabbit Monoclonal Antibody recognizes endogenous levels of total HOXB13 protein.
Species Reactivity: Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly11 of human HOXB13 protein.
Background
HOXB13 is a member of the HOXB cluster which, along with the HOXA, HOXC, and HOXD clusters, governs embryonic patterning along the cranio-caudal axis (1,2). HOXB13 plays a key role in the development of the ventral prostate, where it is expressed highly from the embryonic stage through adulthood (3,4). Research studies have shown that both overexpression and RNA interference can inhibit the growth of prostate cancer cells. HOXB13 can function as a tumor suppressor by negatively regulating growth through repression of TCF4 and androgen receptor (AR) signaling (4,5). However, HOXB13 has also been shown to be overexpressed in more invasive prostate cancers, breast and ovarian cancers, and hepatocellular carcinomas (6-9). A common germline mutation G84E in the HOXB13 protein has recently been found to be associated with significant increased risk of prostate cancer (10). Currently, HOXB13 is being evaluated as a marker for metastatic lesions of prostate origin (11,12).
Alternate Names
homeo box B13; homeobox B13; Homeobox protein Hox-B13; HOXB13; HPC9; HXB13; PSGD
