Description
Product Usage Information
Flow Cytometry (Fixed/Permeabilized): 1:50
Flow Cytometry (Live): 1:50
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Available protocols: Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live)
Specificity / Sensitivity
PTPRJ/DEP-1 (F4G8K) Rabbit Monoclonal Antibody (Alexa Fluor
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to a fragment of the extracellular domain of human PTPRJ/DEP-1 protein.
Background
Protein-tyrosine phosphatase receptor type J (PTPRJ), also known as density-enhanced phosphatase-1 (DEP-1), is a heavily glycosylated, type 1 transmembrane protein. The extracellular region of PTPRJ/DEP-1 contains multiple copies of the fibronectin type III motif, while its intracellular region harbors a single PTP catalytic domain (1). PTPRJ/DEP-1 is expressed at varying levels on multiple hematopoietic cell lineages and has been shown to modulate signaling downstream of the TCR complex (2,3). Research studies have suggested that PTPRJ/DEP-1 also plays a role in non-hematopoietic cell biology through its ability to inhibit cell growth in response to increasing cell density (4). Indeed, PTPRJ/DEP-1-dependent dephosphorylation of mitogenic molecules, such as c-Met and PDGFR-β, has been shown to attenuate signaling downstream of these RTKs (5,6). In many contexts, PTPRJ/DEP-1 functions as a tumor suppressor, and its expression is lost in multiple types of human cancers (7,8).
Alternate Names
CD148; CD148 antigen; Density-enhanced phosphatase 1; DEP-1; DEP1; HPTP eta; HPTPeta; human density enhanced phosphatase-1; protein tyrosine phosphatase receptor type J; protein tyrosine phosphatase, receptor type, J; protein tyrosine phosphatase, receptor type, J polypeptide; Protein-tyrosine phosphatase eta; Protein-tyrosine phosphatase receptor type J; PTPRJ; R-PTP-eta; R-PTP-J; Receptor-type tyrosine-protein phosphatase eta; SCC1; susceptibility to colon cancer 1, mouse, homolog of
