Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
Phospho-cdc2 (Tyr15) Antibody detects endogenous levels of cdc2, CDK2 and CDK5 only when phosphorylated at tyrosine 15. Based on sequence similarity, the antibody may also cross-react with CDK3. This antibody does not cross-react with CDK4, CDK6 or CDK7. This antibody does detect the yeast orthologue of cdc2 (cdc28) when phosphorylated at tyrosine 19.
Species Reactivity: Human, Mouse, Rat, Monkey, D. melanogaster, Xenopus, S. cerevisiae
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr15 of human cdc2. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of cdc2 at Thr161 (1). However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Thr14 and Tyr15 (2). Phosphorylation at Thr14 and Tyr15, resulting in inhibition of cdc2, can be carried out by Wee1 and Myt1 protein kinases (3,4). The cdc25 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of cdc2 (1,5).
Alternate Names
CDC2; CDC28A; CDK1; CDKN1; cell cycle controller CDC2; Cell division control protein 2 homolog; cell division cycle 2; cell division cycle 2, G1 to S and G2 to M; Cell division protein kinase 1; cyclin dependent kinase 1; Cyclin-dependent kinase 1; DKFZp686L20222; MGC111195; p34 protein kinase; P34CDC2
