Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
SEK1/MKK4 Antibody detects endogenous levels of total SEK1/MKK4 protein. This antibody does not cross-react with MEK1, MEK2 or MKK3.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the N-terminus of human SEK1/MKK4. Antibodies are purified by protein A and peptide affinity chromatography.
Background
SAPK/Erk kinase (SEK1), also known as MKK4 or Jun kinase kinase (JNKK), activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflammatory cytokines (1-3). Activation of SEK1 occurs through MEKK phosphorylation of serine and threonine residues at positions 257 and 261, respectively. Like MEK, SEK is a dual-specificity protein kinase that phosphorylates SAPK/JNK at a conserved T*PY* site in its activation loop (4). Phosphorylation by Akt at Ser80 inhibits SEK1 and suppresses stress-activated signal transduction (5).
Alternate Names
c-Jun N-terminal kinase kinase 1; Dual specificity mitogen-activated protein kinase kinase 4; JNK activating kinase 1; JNK-activated kinase 1; JNK-activating kinase 1; JNKK; JNKK1; MAP kinase kinase 4; MAP2K4; MAPK/ERK kinase 4; MAPKK 4; MAPKK4; MEK 4; MEK4; mitogen-activated protein kinase kinase 4; MKK4; MP2K4; PRKMK4; SAPK kinase 1; SAPK/ERK kinase 1; SAPKK-1; SAPKK1; SEK1; SERK1; SKK1; Stress-activated protein kinase kinase 1
