Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
CtIP (D76F7) Rabbit Monoclonal Antibody recognizes endogenous levels of total CtIP protein.
Species Reactivity: Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CtIP protein.
Background
DNA damage checkpoints are critical for regulated repair of damaged DNA and genome maintenance. CtIP/RBBP8 (CtBP-interacting protein), initially characterized as a binding partner for the trancription factor CtBP, has emerged as a regulator of both cell cycle progression and repair of DNA double strand breaks (DSB). Along with the DSB-sensing MRN complex (MRE11-RAD50-NBS1), CtIP functions in the generation of single stranded DNA at DSBs, a process required for signaling to DNA repair machinery (reviewed in 1). CtIP is thought to be critical in the transition between sensing of DSBs and repair by homologous recombination (HR) (2,3). In addition to HR, DSBs can also be repaired through nonhomologous end joining (NHEJ), and CtIP has been shown to have a role in signaling to the NHEJ pathway independently of its function in DSB end resection (4). CtIP is also involved in cellular tolerence of topoisomerase inhibitors camptothecin and etoposide, which are used to treat cancer through their ability to introduce DSBs in cycling cells (5).
Alternate Names
COM1; CtBP-interacting protein; CTIP; DNA endonuclease RBBP8; JWDS; RB binding protein 8, endonuclease; RBBP-8; RBBP8; retinoblastoma binding protein 8; Retinoblastoma-binding protein 8; retinoblastoma-interacting myosin-like; Retinoblastoma-interacting protein and myosin-like; RIM; SAE2; SCKL2; Sporulation in the absence of SPO11 protein 2 homolog
