Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Immunohistochemistry (Paraffin): 1:100 - 1:400
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin)
Specificity / Sensitivity
TRIM21 (D1O1D) Rabbit Monoclonal Antibody recognizes endogenous levels of total TRIM21 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val309 of human TRIM21 protein.
Background
Tripartite motif-containing protein 21 (TRIM21) is a cytosolic antiviral protein that is induced by interferons (IFNs) and acts as a cytosolic IgG receptor (1,2). TRIM21 is an activator of the innate immune system and targets antibody-bound virions to the proteasome via its E3 ubiquitin ligase activity, leading to degradation of the virions in the cytosol (3,4). Interestingly, TRIM21 has also been shown to negatively regulate pathogen-induced IFN production through the polyubiquitin-mediated degradation of multiple targets, including IRF3, IRF7, and DDX41 (5-7).
Alternate Names
52 kDa ribonucleoprotein autoantigen Ro/SS-A; 52 kDa Ro protein; E3 ubiquitin-protein ligase TRIM21; RING finger protein 81; RING-type E3 ubiquitin transferase TRIM21; RNF81; Ro(SS-A); Ro/SSA; Ro/SSA 52kDa; RO52; Sicca syndrome antigen A; Sjoegren syndrome type A antigen; Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro); SS-A; SSA; SSA1; TRIM21; tripartite motif containing 21; tripartite motif-containing 21; Tripartite motif-containing protein 21
