Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:200
Immunohistochemistry (Paraffin): 1:200
Flow Cytometry (Fixed/Permeabilized): 1:400
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 33839 .
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Flow Cytometry (Fixed/Permeabilized)
Specificity / Sensitivity
WHSC1L1 (D4N9N) Rabbit Monoclonal Antibody recognizes endogenous levels of total WHSC1L1 protein, both long and short isoforms.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro117 of human WHSC1L1 protein.
Background
Wolf-Hirschhorn syndrome candidate 1-like protein 1 (WHSC1L1), also known as histone-lysine N-methyltransferase NSD3, is a SET domain-containing histone methyltransferase protein that methylates histone H3 on lysine 4 (H3K4me) and lysine 27 (H3K27me) (1). Methylation of histone H3 lysine 4 is associated with transcriptional activation, while methylation of histone H3 lysine 27 is associated with transcriptional repression. WHSC1L1 can function as an oncogene or a tumor suppressor protein, depending on cell context, and has been shown to regulate expression of a number of genes associated with cell cycle (2). Amplification and/or increased expression of WHSC1L1 in breast, lung, and liver cancer increases growth and survival, and is associated with poor prognosis (2-5). In addition, the NSD3 gene has been found fused with the nuclear pore complex protein NUP98 gene in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL), and fused with the NUT gene in NUT midline carcinomas (6-10).
Alternate Names
DKFZp667H044; FLJ20353; Histone-lysine N-methyltransferase NSD3; KMT3F; KMT3G; MGC126766; MGC142029; NSD3; nuclear receptor binding SET domain protein 3; Nuclear SET domain-containing protein 3; pp14328; Protein whistle; WHISTLE; WHSC1-like 1 isoform 9 with methyltransferase activity to lysine; WHSC1-like protein 1; WHSC1L1; Wolf-Hirschhorn syndrome candidate 1-like 1; Wolf-Hirschhorn syndrome candidate 1-like 1 protein; Wolf-Hirschhorn syndrome candidate 1-like protein 1
