Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
PAXX (D6X7X) Rabbit Monoclonal Antibody recognizes endogenous levels of total PAXX protein.
Species Reactivity: Human, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro195 of human PAXX protein.
Background
DNA double-strand breaks (DSBs) are the most toxic of DNA lesions. They occur in response to genotoxic stress, and they are also an obligate intermediate in the V(D)J recombination events in the immune system. In mammalian cells, the most prominent mechanism by which cells deal with DSBs is known as NHEJ (non-homologous end joining), and involves a core group of proteins that includes Ku, DNA-PK, XRCC4, and XLF (1). PAXX, (Paralog of XRCC4 and XLF, also known as C9orf142 or XLS), is a more recently identified component of the NHEJ machinery whose crystal structure resembles that of XRCC4 (2). PAXX directly interacts with Ku, and promotes accumulation of Ku at DSBs (2,3). Depletion of PAXX impairs cellular DSB repair (2-4,5). Paxx -/- mice develop normally with mild radiosensitivity, but a Paxx/Xlf double knockout is embryonic lethal in mice, indicating synthetic lethality between Paxx and Xlf (6). Paxx/Xlf double knockout have increased apopotosis in post-mitotic motor neurons, as well as impaired development of the adaptive immune system (7).
Alternate Names
C9orf142; CI142; LOC286257; PAralog of XRCC4 and XLF; PAXX; PAXX non-homologous end joining factor; Protein PAXX; XLS; XRCC4-like small protein
