Description
Product Usage Information
Immunofluorescence (Frozen): 1:50
Flow Cytometry (Live): 1:50
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Available protocols: Immunofluorescence (Frozen), Flow Cytometry (Live)
Specificity / Sensitivity
NK1.1/CD161 (E6Y9G) Rabbit Monoclonal Antibody (Alexa Fluor
Species Reactivity: Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse NK1.1/CD161 protein.
Background
CD161/KLRB1 (Killer cell lectin-like receptor subfamily B member 1, also known as CLEC5B and NKR-P1A) is a type II transmembrane protein that is expressed on the majority of Natural Killer (NK) cells, NK T cells, and some T lymphocytes (1). CD161/KLRB1 is also expressed on Th17 cells, promotes their generation, and modulates their function (2). Engagement with its ligand lectin-like transcript 1 (LLT1) inhibits NK cell function, while LLT1 and CD161/KLRB1 interaction in the presence of a TCR signal enhances IFN-gamma production by T cells (3,4). There are several different CD161 isoforms in rodents and some function as activating receptors as well (5,6). There is a family of Klrb1 genes in rodents (7). PK136 antibody recognizes a specific epitope on mouse KLRB1b/c, also referred to as NK1.1, and is commonly used for detection of mouse NK cells in certain mouse strains (CE, B6, NZB, C58, Ma/My, ST, SJL, FVB). However, the epitope is absent in other mouse strains (BALB/c, AKR, CBA, C3H, DBA, 129) (8).
Alternate Names
AI462337; CD161; CD161 antigen-like family member C; CD161c; killer cell lectin-like receptor subfamily A member 1C; killer cell lectin-like receptor subfamily B member 1C; Killer cell lectin-like receptor subfamily B member 1C; Klrb1b; Klrb1c; KLRBC; Ly-5; Ly-55c; Ly-59; Ly5; Ly55; Ly55c; Ly59; lymphocyte antigen 55 complex, locus C; Lymphocyte antigen 55c; lymphocyte antigen 59; natural killer cell receptor protein NKR-P1C; natural killer cell receptor-P1; Natural killer cell surface protein P1-40; natural killer cell-associated antigen 1; NK-; Nk-1; NK-1.; Nk-1.2; NK-RP1; Nk1; Nk1.; NK1.1; Nk1.2; NKR; NKR-; NKR-P1 40; NKR-P1.9; NKR-P1B; NKR-P1C; Nkrp; NKRP1; NKRP140; Nkrp1b; Nkrp1c
