Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
KIF5A (F2W1W) Rabbit Monoclonal Antibody recognizes endogenous levels of total KIF5A protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro817 of human KIF5A protein.
Background
Kinesin superfamily proteins (KIFs) are molecular motors that drive directional, microtubule-dependent intracellular transport of membrane-bound organelles and other macromolecules (e.g., proteins, nucleic acids). The intracellular transport functions of KIFs are fundamentally important for various cellular functions, including mitotic and meiotic division, motility/migration, hormone and neurotransmitter release, and differentiation (1-4). Disruptions to KIF-mediated intracellular transport have been linked with various pathologies, ranging from tumorigenesis to defects in higher-order brain functions such as learning and memory (4-6). KIF5 consists of three family members: KIF5A, KIF5B, and KIF5C (7). KIF5A and KIF5C are specifically expressed in neurons, whereas KIF5B is expressed ubiquitously (7-10). KIF5A has recently also been found to be expressed in astrocyte processes and mutations of the protein have been linked to amyotrophic lateral sclerosis (ALS) (11-13).
Alternate Names
ALS25; D12S1889; KIF5A; KIF5A variant protein; kinesin family member 5A; Kinesin heavy chain isoform 5A; Kinesin heavy chain neuron-specific 1; kinesin, heavy chain, neuron-specific; MY050; NEIMY; Neuronal kinesin heavy chain; NKHC; NKHC1; SPG10
