Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
BRDT (E6T6E) Rabbit Monoclonal Antibody recognizes endogenous levels of total BRDT protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human BRDT protein.
Background
Testis-specific bromodomain-containing protein (BRDT) is a member of the bromodomains and extra terminal (BET) family of proteins, which also includes BRD2, BRD3, and BRD4 (1-3). BET family proteins contain two tandem bromodomains and an extra terminal (ET) domain, and bind acetyl lysine residues (3). BRDT is expressed in the testes and is essential for male germ cell differentiation, acting as a master regulator of meiotic divisions and post-meiotic genome repackaging (2,4). JQ1 treatment of BRDT has been explored as a potential male contraceptive, as JQ1-treated mice exhibit reduced spermatozoa number and motility without affecting hormone levels (5).
Alternate Names
BRD6; BRDT; bromodomain testis associated; Bromodomain testis-specific protein; bromodomain, testis-specific; Cancer/testis antigen 9; CT9; RING3-like protein; SPGF21; testis-specific bromodomain protein
