Description
Product Usage Information
Important! This control antibody must be diluted to the same concentration (not dilution) as the specific antibody used in analysis. Higher background fluorescence may result if excessive amounts of isotype control are used. For protocol details, please reference the product page for the specific antibody used in analysis.
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Specificity / Sensitivity
Rabbit (DA1E) IgG Isotype Control F(ab')
Background
Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to F receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody. Human F receptors exhibit strong cross-reactivity with rabbit IgG, and are expressed at high levels on cells of myeloid lineage (e.g., monocytes and myeloid-derived cell lines). Fluorescent F(ab') fragment conjugates are ideal for use in flow cytometry assays on cells containing F receptors, and eliminate the need for an F blocking step prior to antibody incubation. Cell Signaling Technology employs strict quality control procedures to ensure that each conjugate provides optimal specificity and fluorescence.
