Description
Product Usage Information
Flow Cytometry (Fixed/Permeabilized): 1:50
Flow Cytometry (Live): 1:50
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Available protocols: Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live)
Specificity / Sensitivity
CD81 (D5O2Q) Rabbit Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total CD81 protein.
Species Reactivity: Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro184 of mouse CD81 protein.
Background
CD81 belongs to the tetraspanin family, which is characterized by four transmembrane domains, one short extracellular domain (ECL1), and one long extracellular domain (ECL2) (1-3). Tetraspanins interact with a variety of cell surface proteins and intracellular signaling molecules in specialized tetraspanin enriched microdomains (TEMs) where they mediate a range of processes including adhesion, motility, membrane organization, and signal transduction (3). CD81, like other tetraspanins, is enriched in exosomes (4). Many research studies demonstrate a role for CD81 in lymphocyte signaling (5-7). CD81 is also a well-characterized receptor for Hepatitis C Virus and facilitates the entry of the virus into target cells (8, 9).
Alternate Names
26 kDa cell surface protein TAPA-1; Cd81; CD81 antigen; Tap; Tapa-1; Tapa1; Target of the antiproliferative antibody 1; Tsp; Tspan28
