Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
Argonaute 1 Antibody detects endogenous levels of total Argonaute 1 protein.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys400 of human Argonaute 1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Small non-coding RNAs are important regulators of gene expression in higher eukaryotes (1,2). Several classes of small RNAs, including short interfering RNAs (siRNAs) (3), microRNAs (miRNAs) (4), and Piwi-interacting RNAs (piRNAs) (5), have been identified. miRNAs are about 21 nucleotides in length and have been implicated in many cellular processes, such as development, differentiation, and stress response (1,2). miRNAs regulate gene expression by modulating mRNA translation or stability (2). miRNAs function together with the protein components in the complexes called micro-ribonucleoproteins (miRNPs) (2). Among the most important components in these complexes are Argonaute proteins (1,2). There are four members in the mammalian Argonaute family, and only Argonaute 2 (Ago2) possesses the Slicer endonuclease activity (1,2). Argonaute proteins participate in the various steps of miRNA-mediated gene silencing, such as repression of translation and mRNA turnover (1).
Alternate Names
AGO1; argonaute 1; argonaute 1, RISC catalytic component; Argonaute RISC catalytic component 1; argonaute RISC component 1; Argonaute1; DKFZp686M13167; eIF-2C 1; EIF2C; eIF2C 1; EIF2C1; Eukaryotic translation initiation factor 2C 1; eukaryotic translation initiation factor 2C, 1; GERP95; Golgi Endoplasmic Reticulum protein 95 kDa; hAgo1; Protein argonaute-1; Putative RNA-binding protein Q99; Q99
