Description
Product Usage Information
Western Blotting: 1:1000
Simple Western™: 1:50 - 1:250
Immunoprecipitation: 1:200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 63108 .
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
15-PGDH/HPGD (E9H7W) Rabbit Monoclonal Antibody recognizes endogenous levels of total 15-PGDH/HPGD protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human 15-PGDH/HPGD protein.
Background
15-hydroxyprostaglandin dehydrogenase (15-PGDH or HPGD) catalyzes the rate-limiting step of prostaglandin E2 (PGE2) degradation (1). Its inhibition was shown to potentiate tissue regeneration in various mouse organs (2). Increased 15-PGDH/HPGD levels are a hallmark of aged skeletal muscles. 15-PGDH/HPGD inhibition in aged mouse muscles leads to elevated PGE2 levels and boosts mitochondrial biogenesis and function, enhancing muscle mass and strength (3). Studies show that loss of 15-PGDH/HPGD also leads to increased hematopoietic output and enhanced gastrointestinal fitness in aged mice, indicating a critical role of this enzyme in the regulation of tissue regeneration during aging (1). In addition, PPARγ upregulates15-PGDH/HPGD expression in the regulatory T cells in visceral adipose tissue, suppressing conventional T cell activation and proliferation (4).
Alternate Names
15-hydroxyprostaglandin dehydrogenase; 15-hydroxyprostaglandin dehydrogenase (NAD(+)); 15-hydroxyprostaglandin dehydrogenase [NAD(+)]; 15-hydroxyprostaglandin dehydrogenase [NAD+]; 15-PGDH; HPGD; hydroxyprostaglandin dehydrogenase 15-(NAD); NAD+-dependent 15-hydroxyprostaglandin dehydrogenase; PGDH; PGDH1; PHOAR1; Prostaglandin dehydrogenase 1; SDR36C1; Short chain dehydrogenase/reductase family 36C member 1; short chain dehydrogenase/reductase family 36C, member 1
