Description
Product Usage Information
Western Blotting: 1:2000
Immunoprecipitation: 1:50
Flow Cytometry (Fixed/Permeabilized): 1:400
Peptide ELISA (DELFIA): 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Fixed/Permeabilized)
Specificity / Sensitivity
Phospho-Tyrosine Mouse Monoclonal Antibody (P-Tyr-102) is a high affinity IgG1 monoclonal antibody. ELISAs using a wide variety of phospho-peptides show that P-Tyr-102 binds phospho-Tyr in a manner largely independent of the surrounding amino acid sequence. 2D gel western blot analysis of pervanadate-treated cell extracts also shows that P-Tyr-102 interacts with a broad range of tyrosine-phosphorylated proteins. P-Tyr-102's fine specificity in terms of the sequence context in which it can recognize phospho-tyrosine seems to differ slightly from that of Phospho-Tyrosine Mouse Monoclonal Antibody (P-Tyr-100) #9411. This antibody does not recognize peptides containing phospho-Ser or phospho-Thr.
Species Reactivity: All Species Expected
Source / Purification
Monoclonal antibody is produced by immunizing animals with synthetic phospho-Tyr-containing peptides.
Background
Tyrosine phosphorylation plays a key role in cellular signaling (1). Research studies have shown that in cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology are exceptionally sensitive tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.
