Description
Product Usage Information
Immunoprecipitation: 1:20
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at -20°C. Do not aliquot the antibodies.
Protocol
Available protocols: Immunoprecipitation
Specificity / Sensitivity
Phospho-Tyrosine Mouse Monoclonal Antibody (P-Tyr-100) (Sepharose
Species Reactivity: All Species Expected
Source / Purification
Monoclonal antibody is produced by immunizing animals with phospho-Tyr-containing peptides.
Background
Tyrosine phosphorylation plays a key role in cellular signaling (1). Research studies have shown that in cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology are exceptionally sensitive tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.
