Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
GRB7 (E7R8V) Rabbit Monoclonal Antibody recognizes endogenous levels of total GRB7 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg29 of human GRB7 protein.
Background
GRB7 belongs to the GRB7 family of adaptor proteins that mediate cell surface and cytosolic tyrosine kinases to downstream signaling pathways (1-3). The C-terminal SH2 domain of GRB7 has been shown to be important for its interaction with various upstream molecules such as ERBB receptor tyrosine kinases, FAK, EphB1, and SHC. The protein has an RA, CAM-BD, and PH domain located at the central region (GM region) of its sequences to mediate downstream Ras, PI3K/Akt, ERK, and Calmodulin signaling (3,4). Overexpression of GRB7 promotes cancer proliferation and migration, and disruption of GRB7 function inhibits its effect on cancer growth, invasion, and metastasis (3-5).
Alternate Names
B47; Epidermal growth factor receptor GRB-7; GRB7; GRB7 adapter protein; growth factor receptor bound protein 7; Growth factor receptor-bound protein 7
