Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
Caspase-9 Antibody detects endogenous levels of both full length mouse caspase-9 (49 kDa) and the large fragment of mouse caspase-9 resulting from cleavage at aspartic acid 353 (37 kDa) and/or aspartic acid 368 (39 kDa). This antibody does not cross-react other caspases.
Species Reactivity: Mouse
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the cleavage site of mouse caspase-9. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf-1. Apaf-1 mediated activation of caspase-9 involves intrinsic proteolytic processing, resulting in cleavage at Asp315 and producing a p35 subunit. Another cleavage occurs at Asp330, producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10).
Alternate Names
AI115399; APAF-3; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; AW493809; Casp; CASP-9; Casp9; caspase 9; Caspase-9; Caspase-9 subunit p10; Caspase-9 subunit p35; Caspase9; ICE-; ICE-LAP6; ICE-like apoptotic protease 6; Mch6; OTTMUSP00000010525
