Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
CPSF1 (E5P8C) Rabbit Monoclonal Antibody recognizes endogenous levels of total CPSF1 protein.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1081 of human CPSF1 protein.
Background
Cleavage and polyadenylation of pre-mRNA is regulated by a core group of proteins called the cleavage and polyadenylation specificity factors (CPSF) (1,2). The CPSF proteins interact with poly(A) polymerase (PAP) to recognize the AAUAAA sequence motif and add poly(A). CPSF can also interact with downstream cleavage factors to precisely cleave the 3' end of pre-mRNA (2,3). CPSF is brought to 3' ends by the carboxy-terminal domain of the Rpb1 subunit of the RNA Polymerase II complex, where it dissociates and initiates polyadenylation (4). CPSF has been shown to have numerous interactions with viral proteins. The influenza NS1 viral protein binds to CPSF4 to prevent 3' end processing of viral RNAs, inhibiting nuclear export (5).
Alternate Names
cleavage and polyadenylation specific factor 1; cleavage and polyadenylation specific factor 1, 160kDa; Cleavage and polyadenylation specificity factor 160 kDa subunit; Cleavage and polyadenylation specificity factor subunit 1; CPSF 160 kDa subunit; CPSF1; CPSF160; HSU37012; MYP27; P/cl.18; polyadenylation specificity factor
