Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Immunofluorescence (Immunocytochemistry): 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
SMG-1 (V72) Antibody detects endogenous levels of total SMG-1 protein. This antibody cross-reacts with a 100 kDa band of unknown origin. By confocal immunofluorescence, the antibody labels the mitochondria of U-2 OS cells (7).
Species Reactivity: Human, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human SMG-1.
Background
SMG-1 is a member of the phosphoinositide 3-kinase-related kinase (PIKK) family, which includes ATM, ATR, mTOR, DNA-PKcs, and TRRAP (1,2). Activated by DNA damage, SMG-1 has been shown to phosphorylate p53 and hUpf1 (SMG-2) (1-4). hUpf1 is a subunit of the surveillance complex that allows degradation of messenger RNA species containing premature termination codons (PTCs). This process, known as nonsense-mediated mRNA decay (NMD), prevents the translation of truncated forms of proteins that may result in gain of function or dominant negative species. NMD occurs under normal cellular conditions as well as in response to damage (5,6). SMG-1 has also been shown to affect cell death receptor signaling and to protect cells from extrinsically induced apoptotic cell death (7).
Alternate Names
61E3.4; ATX; hSMG-1; KIAA0421; Lambda-interacting protein; Lambda/iota protein kinase C-interacting protein; LIP; phosphatidylinositol 3-kinase-related kinase; phosphatidylinositol 3-kinase-related protein kinase; PI-3-kinase-related kinase SMG-1; Serine/threonine-protein kinase SMG1; SMG-1; smg-1 homolog, phosphatidylinositol 3-kinase-related kinase; SMG1; SMG1 homolog, phosphatidylinositol 3-kinase-related kinase (C. elegans); SMG1 nonsense mediated mRNA decay associated PI3K related kinase; SMG1 phosphatidylinositol 3-kinase-related kinase
