Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
IHC Leica Bond: 1:100
Immunohistochemistry (Paraffin): 1:250
Immunofluorescence (Immunocytochemistry): 1:500
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 59728 .
Protocol
Available protocols: Western Blotting, Immunoprecipitation, IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
CD79B (D7V2F) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD79B protein. This antibody is predicted to detect multiple isoforms of CD79B.
Species Reactivity: Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val222 of human CD79B protein.
Background
The B-cell antigen receptor (BCR) is composed of membrane immunoglobulin molecules non-covalently associated with the heterodimeric signaling component, CD79A and CD79B (also known as Igα and Igβ, respectively) (1,2). The presence of this receptor complex is essential for B cell development and function (3). Following antigen binding, CD79A/CD79B heterodimers are phosphorylated and initiate intracellular signaling through Src family kinases, Lyn, Blk, and Fyn, as well as Syk and Btk tyrosine kinases (4,5). The complexity of BCR signaling results in a variety of distinct cellular functions, such as proliferation, tolerance, apoptosis, and differentiation (6). BCR-antigen ligation also leads to internalization of the complex, trafficking to late endosomes, and antigen presentation in major histocompatibility molecules on the B cell surface (7,8). CD79B enhances the phosphorylation of CD79A (9). Alternatively spliced transcript variants encoding different isoforms of CD79B have been identified (10). CD79B is widely expressed on B cell malignancies and may serve as a target for therapeutic intervention (11,12).
Alternate Names
AGM6; B-cell antigen receptor complex-associated protein beta chain; B-cell-specific glycoprotein B29; B29; CD79B; CD79B antigen; CD79b antigen (immunoglobulin-associated beta); CD79b molecule; CD79b molecule, immunoglobulin-associated beta; Ig-beta; IGB; Immunoglobulin-associated B29 protein
