Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
BCL7A Antibody recognizes endogenous levels of total BCL7A protein. This antibody does not cross-react with other BCL7 proteins.
Species Reactivity: Human, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val168 of human BCL7A protein. Antibodies are purified by peptide affinity chromatography.
Background
The BCL7 family of proteins consisting of BCL7A, BCL7B, and BCL7C are part of the mammalian SWI/SNF complex (1,2). The BCL7 family has tumor suppressive properties, and differential expression is correlated with poor prognosis of multiple cancer types (2-6). BCL7A was first identified as gene invoked in a MYC-BCL7A fusion in high-grade B-cell non-Hodgkin lymphoma (7). BCL7A also contributes to the function of Purkinje cells (8). BCL7B is deleted in Williams-Beuren syndrome and has been found to play a role in Wnt signaling (9,10).
Alternate Names
B-cell CLL/lymphoma 7 protein family member A; B-cell CLL/lymphoma 7A; B-cell CLL/lymphoma-7; BAF chromatin remodeling complex subunit BCL7A; BCL tumor suppressor 7A; Bcl-7A; BCL7; BCL7A; BCL7A, BAF complex component
