Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
Rab35 Antibody detects endogeneous levels of total Rab35 protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp124 of human Rab35 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Rab35 belongs to the small GTPase superfamily. By interacting with its specific GEF or GAP, Rab35 regulates cargo-specific endocytosis at the vesicle recycling step or mediates exocytosis at the exosome docking/tethering step (1-4). During cytokinesis, Rab35 interacts with OCRL phosphatase and is essential for maintaining intercellular bridge stability and abscission by controlling the concentration of phosphatidylinositol 4,5-bisphosphate (PIP2) and SEPT2 localization at the intercellular bridge (5,6). Rab35 also plays a role in actin assembly and the recruitment of Cdc42 and Rac1 to the site of filopodium by its direct interaction with actin-bundling protein fascin and actin-binding protein connecdenn 3 (7-9).
Alternate Names
GTP-binding protein RAY; H-ray; RAB1C; RAB35; RAB35, member RAS oncogene family; Ras-related protein Rab-1C; ras-related protein rab-1c (GTP-binding protein ray); Ras-related protein Rab-35; RAY
