Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody .
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
Ykt6 Antibody recognizes endogenous levels of total Ykt6 protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro133 of human Ykt6 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Synaptobrevin-2 homolog Ykt6 is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein family. SNARE proteins represent the core membrane fusion machinery that drives membrane fusion in the cell. Ykt6 is an unusual SNARE as it lacks a transmembrane domain but uses farnesylation and palmitoylation as membrane anchors to mediate SNARE-dependent membrane fusion (1). Ykt6 plays a key role in ER-Golgi trafficking but can also facilitate other organelle transport, including intra-Golgi, endosome-Golgi, and autophagosome formation trafficking. Ykt6 is also involved in trafficking of proteins to lysosomal compartments from the Golgi under normal conditions and in response to cellular stress. α-Synuclein, a protein that accumulates and is genetically linked to Parkinson's disease and dementia with Lewy bodies, associates with and deactivates Ykt6 (2). Deactivation of Ykt6 by α-Synuclein alters normal α-Synuclein proteostasis and may represent a cellular mechanism by which α-Synuclein accumulation occurs.
Alternate Names
R-SNARE; SNARE protein Ykt6; Synaptobrevin homolog YKT6; YKT6; YKT6 v-SNARE homolog; YKT6 v-SNARE homolog (S. cerevisiae); YKT6 v-SNARE protein; YKT6, S. cerevisiae, homolog of
