Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
ACAD9 Antibody recognizes endogenous levels of total ACAD9 protein.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val512 of human ACAD9 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The mitochondrial flavoenzymes acyl-CoA dehydrogenases (ACADs) catalyze the α,β dehydrogenation of acyl-CoA esters (1). One of these enzymes, ACAD9, is essential for assembly of oxidative phosphorylation complex I (2). Studies have shown that ACAD9 gene mutations cause Complex I deficiency (2,3).
Alternate Names
ACAD-9; ACAD9; Acyl-CoA dehydrogenase family member 9; Acyl-CoA dehydrogenase family member 9, mitochondrial; acyl-Coenzyme A dehydrogenase family, member 9; Complex I assembly factor ACAD9, mitochondrial; FLJ23533; MC1DN20; MGC14452; NPD002; very-long-chain acyl-CoA dehydrogenase VLCAD
