Description
Product Usage Information
Western Blotting: 1:1000
Simple Western™: 1:50 - 1:250
Immunoprecipitation: 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
MAD2L2 (F9H9U) Rabbit Monoclonal Antibody recognizes endogenous levels of total MAD2L2 protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val203 of human MAD2L2 protein.
Background
Mitotic arrest deficient 2 like 2 (MAD2L2), also known as REV7, is a Mad family mitotic spindle assembly checkpoint protein that regulates progression into anaphase of mitosis (1), and functions in translesion DNA synthesis and DNA double-strand break (DSB) repair (2). MAD2L2 expression is high in human cancer, and expression levels are correlated with prognosis. MAD2L2 expression may predict the effectiveness of chemotherapy and PARP inhibitors, and MAD2L2 inhibitors may enhance the effects of these treatments (3).
Alternate Names
FANCV; hREV7; MAD2 (mitotic arrest deficient, yeast, homolog)-like 2; MAD2 mitotic arrest deficient-like 2; MAD2 mitotic arrest deficient-like 2 (yeast); MAD2-like protein 2; MAD2B; MAD2L2; MD2L2; mitotic arrest deficient 2 like 2; Mitotic arrest deficient 2-like protein 2; mitotic arrest deficient homolog-like 2; Mitotic spindle assembly checkpoint protein MAD2B; polymerase (DNA-directed), zeta 2, accessory subunit; POLZ2; REV7; REV7 homolog
