Description
Product Usage Information
Western Blotting: 1:1000
Simple Western™: 1:10 - 1:50
Immunofluorescence (Immunocytochemistry): 1:200 - 1:800
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
TOM70 (F4B1Y) Mouse Monoclonal Antibody recognizes endogenous levels of total TOM70 protein. This antibody non-specifically stains select nuclei in mouse tissue by immunofluorescence.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser75 of human TOM70 protein.
Background
Mitochondria play a central role in cellular energy metabolism and are essential organelles in eukaryotes. In humans, 13 proteins are encoded by the mitochondrial genome, while the vast majority of mitochondrial proteins are encoded by the nuclear genome. As a result, most mitochondrial proteins are synthesized as precursors in the cytoplasm and imported across mitochondrial membranes by one or more translocase protein complexes (1). The translocase of the outer mitochondrial membrane (TOM complex) facilitates the import of proteins through the outer mitochondrial membrane, while the complementary translocase of the inner membrane (TIM complex) is responsible for protein transport to the mitochondrial matrix. The TOM complex consists of the receptors TOM20, TOM22, TOM70, and the channel-forming protein TOM40 (1).
Alternate Names
FLJ90470; KIAA0719; Mitochondrial import receptor subunit TOM70; Mitochondrial precursor proteins import receptor; TOM70; TOMM70; TOMM70A; Translocase of outer membrane 70 kDa subunit; translocase of outer mitochondrial membrane 70; translocase of outer mitochondrial membrane 70 homolog A; translocase of outer mitochondrial membrane 70 homolog A (S. cerevisiae); Translocase of outer mitochondrial membrane protein 70
