Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
COMMD3 (E2X1V) Rabbit Monoclonal Antibody recognizes endogenous levels of total COMMD3 protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys187 of human COMMD3 protein.
Background
The COMMD/CCDC93/CCDC22 (CCC) protein complex contains any one of ten COMMD proteins. The CCC complex, which is a subcomplex of the commander complex, plays a key role in protecting the stability and promoting endosomal recycling of cell surface transmembrane proteins (1-3). Indeed, research studies have demonstrated that COMMD3 regulates endosomal trafficking of HER2 (4). In line with its roll in modulating endosomal trafficking of proteins, multiple functional genetic screens identified COMMD3 as a SARS-CoV-2 proviral host factor (5,6).
Alternate Names
BUP; C10orf8; COMD3; COMM domain containing 3; COMM domain-containing protein 3; COMMD3; DKFZp686K0399; FLJ45471; Protein Bup; Protein PIL
