Description
Product Usage Information
Immunofluorescence (Frozen): 1:50 - 1:200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Immunofluorescence (Frozen)
Specificity / Sensitivity
Doublecortin (F6K9E) Feline Chimeric Monoclonal Antibody recognizes endogenous levels of total doublecortin protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
This recombinant chimeric antibody is engineered from Doublecortin (F6K9E) Rabbit mAb #56130 according to animal-free protocols. This chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a feline-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies. The parent antibody, Doublecortin (F6K9E) Rabbit mAb #56130, is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human doublecortin protein.
Background
Mutations in doublecortin ( ) cause Lissencephaly (smooth brain), a neuronal migration disorder characterized by epilepsy and mental retardation (1). Doublecortin is a microtubule-associated protein that stabilizes and bundles microtubules. A conserved doublecortin domain mediates the interaction with microtubules, and interestingly most missense mutations cluster in this domain (2). Kinases JNK, CDK5, and PKA phosphorylate doublecortin. JNK phosphorylates Thr321, Thr331, and Ser334 while PKA phosphorylates Ser47 and CDK5 phosphorylates Ser297 (3-5). Phosphorylation of Ser297 lowers the affinity of doublecortin to microtubules. Furthermore, mutations of Ser297 result in migration defects (5).
Alternate Names
DBCN; DC; DCX; doublecortex; doublecortin; Doublin; Lis-X; Lissencephalin-X; LISX; Neuronal migration protein doublecortin; SCLH; XLIS
