Description
Product Usage Information
The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:800 should yield acceptable results for immunofluorescent assays. Texas Red has relatively low photostability. Usage of an antifade reagent is recommended to preserve signal intensity.
Storage
Supplied lyophilized with a formulation of 0.02 M potassium phosphate, 0.15 M sodium chloride (pH 7.2), 10 mg/mL BSA, and 0.01% azide at 4ºC. Reconstitute lyophilized material with 1.0 mL dH2O and centrifuge until clear. The product is stable for six weeks at 4ºC as an undiluted liquid. Solutions should be used within 12 months. Aliquot to avoid multiple freeze/thaw cycles.
Specificity / Sensitivity
Goat Anti-Feline IgG, Fc Fragment Antibody (Texas Red Conjugate) reacts with the Fc portion of feline IgG. This antibody conjugate does not react with the Fab portion of feline IgG.
Species Reactivity: Feline
Background
This product is optimized for use as a secondary antibody in immunofluorescent applications. Fluorescent anti-species IgG conjugates are ideal for immunofluorescence. Cell Signaling Technology applies strict quality control procedures to ensure that each conjugate provides optimal specificity and fluorescence.
