Description
MabSelect SuRe resin properties This resin for monoclonal antibody (mAb) purification contains a rigid, high-flow agarose matrix that delivers excellent pressure/flow properties. Like many of Cytiva's protein A chromatography resins, MabSelect SuRe™ uses oriented ligand coupling for excellent binding capacity. It exhibits low nonspecific protein binding, thereby decreasing impurity levels in the eluate pool. Benefits of alkali-tolerant protein A ligand MabSelect SuRe™ resin features an engineered, alkali-tolerant rProtein A ligand for mAb purification. The ligand provides greater stability than conventional protein A affinity resins in the alkaline conditions used in cleaning-in-place (CIP) and sanitization protocols. The resin can withstand rigorous CIP and sanitization procedures with 0.1 to 0.5 M NaOH. The enhanced alkali stability of this resin improves process economy and product quality; cleaning can be performed with cost-effective reagents such as sodium hydroxide, eliminating the need for expensive and hazardous cleaning agents such as Gua-HCl. Generic elution conditions for different mAbs MabSelect SuRe™ resin offers generic elution conditions for different monoclonal antibodies, which is an advantage when designing purification platform processes. This feature is also correlated to the construction of the rProtein A ligand, which has a reduced interaction with the VH3 (Fab) domain present in human antibodies. Compatible empty columns – bioprocess scale For process-scale applications, Cytiva recommends AxiChrom™ chromatography columns for the MabSelect™ family of products. Convenient prepacked columns – research scale This chromatography resin is also available in the following formats: HiTrap™ MabSelect SuRe™: convenient for easy use with a syringe, peristaltic pump, and chromatography system HiScreen™ MabSelect SuRe™ for method and process development PreDictor™ MabSelect SuRe™ 96-well filter plate for high-throughput screening or process development PredDictor™ RoboColumns MabSelect™– miniaturized column format for high-throughput process development Convenient prepacked columns – bioprocess scale Prepacked ReadyToProcess™ columns are available, eliminating the need for column packing and cleaning validation. Learn about prepacked HiScreen MabSelect SuRe , HiTrap MabSelect SuRe , ReadyToProcess MabSelect SuRe columns , PreDictor MabSelect SuRe or PreDictor Robocolumns MabSelect SuRe
