DWK, 353262, WHEATON® Glass Shake Flask, With Three Baffles, 500 mL

Oxygen intake is often the limiting factor for cell growth in the cultivation of microorganisms in Erlenmeyer flasks on a vibrating board or orbital shaker. The movement causes a liquid sickle to form when using Erlenmeyer flasks on a vibrator. The size of the sickle depends on the speed of the board and the vibration diameter. The greater the surface area of the contents, the greater the gas exchange area and therefore the potential oxygen intake. The speed and the associated oxygen intake can, however, only be increased to a limited extent. The shake flask with baffles on the bottom disrupts the laminar flow and produces a turbulent flow. The surface area of the liquid and the gas exchange area are increased, thereby increasing the oxygen intake. Laboratory trials have demonstrated that the oxygen intake is doubled by the baffles compared to a standard Erlenmeyer flask.