Merck, 492016, NP-40 Alternative

General description
A non-ionic surfactant used in the isolation and purification of functional membrane protein complexes and in two-dimensional (2D) gel electrophoresis. Similar in usage to NP-40 and TRITON™ X-100 Detergent(Cat. No. 648462).
Application
NP-40 Alternative has been used:
as a component of the lysis buffer for solubilizing proteins from transfected HEK293 cells.
as a component of the wash buffer for efficient solubilization of biotinylated plasma proteins in plasma protein profiling and chromatin immunoprecipitation (ChIP).
for the purification of doublet microtubules from cilia for single particle cryo-electron microscopy (cryo-EM).

Other Notes
Ripoche, P., et al. 1982. Biochim. Biophys. Acta693, 497.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
Disclaimer
Toxicity: Irritant (B)