Merck, 577433-U, BIOshell™ IgG 1000 Å Diphenyl, 2.7 μm HPLC Guard Cartridge
Size:3 ea, BIOshell™ IgG 1000 Å Diphenyl, 2.7 μm HPLC Column, pack of 3 ea, cartridge L × I.D. 0.5 cm × 4.6 mm
Catalog Number: 577433-U
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Category
Pack Size3 ea
Description
General description
BIOshell™ IgG 1000 Å Diphenyl is a high-speed, high-performance liquid chromatography column based on a new wide-pore (1000 Å) Fused-Core® particle design. The particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.5 μm thin porous shell and the small overall particle size of 2.7 μm. The densely bonded, extensively endcapped diphenylmethyl bonded phase of BIOshell™ IgG 1000 Å Diphenyl provides a stable, reversed-phase packing that can be used for separating high molecular weight compounds, such as proteins.
Application
UHPLC Analysis of Reduced IgG1 on BIOshell™ IgG 1000 Å Diphenyl
UHPLC Analysis of IgG2 on BIOshell™ IgG 1000 Å C4, C18, and Diphenyl, 2.7 μm
Analysis Note
Target analytes include: monoclonal antibodies, antibody-drug conjugates, antibody fragments and large proteins with molecular weights up to 500 kDa.
Legal Information
BIOshell is a trademark of Sigma-Aldrich Co. LLC
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
BIOshell™ IgG 1000 Å Diphenyl is a high-speed, high-performance liquid chromatography column based on a new wide-pore (1000 Å) Fused-Core® particle design. The particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.5 μm thin porous shell and the small overall particle size of 2.7 μm. The densely bonded, extensively endcapped diphenylmethyl bonded phase of BIOshell™ IgG 1000 Å Diphenyl provides a stable, reversed-phase packing that can be used for separating high molecular weight compounds, such as proteins.
Application
UHPLC Analysis of Reduced IgG1 on BIOshell™ IgG 1000 Å Diphenyl
UHPLC Analysis of IgG2 on BIOshell™ IgG 1000 Å C4, C18, and Diphenyl, 2.7 μm
Analysis Note
Target analytes include: monoclonal antibodies, antibody-drug conjugates, antibody fragments and large proteins with molecular weights up to 500 kDa.
Legal Information
BIOshell is a trademark of Sigma-Aldrich Co. LLC
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
Specifications
General
| UNSPSC Code | 41115700 |
|---|---|
| Web Name Suffix Override | L × I.D. 0.5 cm × 4.6 mm, HPLC Guard Cartridge, pack of 3 ea |
| Web Name Override | BIOshell™ IgG 1000 Å Diphenyl (2.7 μm) HPLC Columns |
| NACRES | SB.52 |
| material | stainless steel column |
| agency | suitable for USP L11 |
| feature | endcapped |
| packaging | pack of 3 ea |
| extent of labeling | 1.0% loading |
| technique(s) | HPLC: suitable |
| cartridge L × I.D. | 0.5 cm × 4.6 mm |
| surface area | 22 m2/g |
| matrix | Fused-Core® particle platform |
| matrix active group | bonding phase, Diphenyl (diphenylmethyl) |
| particle size | 2.7 μm |
| pore size | 1000 Å pore size |
| operating pH range | 2-9 |
| separation technique | reversed phase |
| product line | BIOshell™ |
