Description
General description
A reagent for the reversible labeling of peptides with biotin [¹]. Labeling of the N-terminal amine of resin-bound peptides is carried out by incubating overnight with excess reagent in DMF. The biotinylated peptide is obtained following TFA cleavage. Removal of the biotin label is effected by treatment with 5% aqueous hydrazine.
Associated Protocols and Technical Articles
Purification of Peptides using Chemoselective Tags
Literature references
[¹] B. Kellam, et al. (1997) Tetrahedron Lett., 38, 5391
Analysis Note
Color (visual): white to slight yellow to beige
Appearance of substance (visual): powder
Identity (IR): passes test
Purity (TLC(011A)): ≥ 98 %
Assay (HPLC, area%): ≥ 98.0 % (a/a)
To see the solvent systems used for TLC of Novabiochem® products please click here.
Other Notes
Replaces: 01-63-0108
Legal Information
Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany
