Description
General description
Multiple epidermal growth factor-like domains protein 10 (Megf10) is a type 1 transmembrane protein with multiple EGF-like extracellular domains. Megf10 is highly expressed in the brain, and plays an important role in phacocytosis of apoptotic cells. This role may be performed through the interaction of Megf10 with the ATP-binding cassette transporter (ABCA1) protein. Previous studies have suggested that Megf10 may be involved in the uptake of amyloid β peptides in the brain. Megf10 may therefore play a role Alzheimer’s disease.
~130 kDa observed
Immunogen
Epitope: Cytoplasmic domain
Histidine-tagged recombinant protein corresponding to the cytoplasmic domain of mouse Megf10.
Application
Detect the Megf10 protein using this Anti-Megf10 validated for use in WB & IF.
Immunofluorescence Analysis: A representative lot was used to detect Megf10 in P7 caudal brain tissue and retina cells by an independent laboratory. (Image courtesy form the laboratory of Dr. Joshua Sanes, Harvard University).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Biochem/physiol Actions
Other homologies: Rat (98% sequence homology) and Human (92% sequence homology).
This antibody recognizes Megf10 at the cytoplasmic domain.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Mouse retina tissue lysate
Evaluated by Western Blotting in Mouse Brain tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Megf10 in Mouse Brain tissue lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
