Description
General description
Atg4D (autophagy-related protein 4D) is a member of the Atg4 family of C54 endopeptidases that are required for the synthesis of autophagosomes. Atg4D is found to be cleaved by caspase-3 into a more active form where it can interact with the autophagosome marker Atg8. Atg4D is also known to be associated with mitochondrial dysfunction and the induction of apoptosis.
~56 kDa observed.
The calculated molecular weight is 53 kDa; however, Atg4D has been shown as a ~55 kDa band in western blots (Li, M., et al. (2011). J Biol Chem. 286(9):7327-7338.)
Immunogen
Epitope: N-terminus
KLH-conjugated linear peptide corresponding to the N-terminus of human Atg4D.
Application
Anti-Atg4D Antibody is an antibody against Atg4D for use in WB, IH(P).
Immunohistochemistry (Paraffin) Analysis: A 1:200 dilution of a previous lot detected Atg4D in human testicular cancer tissue.
Western Blot Analysis: 1 µg/mL from a representative lot detected Atg4D on 10 µg of human fetal skeletal muscle tissue, NIH/3T3, and L6 cell lysates.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Biochem/physiol Actions
This antibody recognizes Atg4D at the N-terminus.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
A431 cell lysate
Evaluated by Western Blot in A431 cell lysate.
Western Blot Analysis: 0.05 µg/mL of this antibody detected Atg4D in 10 µg of A431 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
