Description
General description
The protein Death-associated protein 1 or also known as DAP-1/DAP1 and encoded by the gene DAP/DAP1 is a negative regulator of autophagy. The link of DAP1 to autophagy was recognized because in DAP1 knockdowns there was enhanced autophagic flux and DAP1 displayed a rapid decline in its phosphorylation in response to amino acid starvation, a key inducer of autophagy. Mapping of the phosphorylation sites and analysis of phosphorylation mutants indicated that DAP1 is functionally silenced in growing cells through mTOR-dependent phosphorylation on Ser3 and Ser51. Inactivation of mTOR during starvation caused a rapid reduction in these phosphorylation sites and converted the protein into an active suppressor of autophagy. These results are consistent with a "Gas and Brake" model in which mTOR inhibition also controls a buffering mechanism that counterbalances the autophagic flux and prevents its over activation under nutrient deprivation. DAP1 is also involved in mediating interferon gamma induced cell death, which appears to often be mediated by autophagy, and recently it has been shown to be important in regulating myogenesis and the apoptosis of satellite cells during development. DAP1/Death-associated protein 1 is regulated by phosphorylation; phosphorylation by MTOR inhibits the suppressive activity of DAP toward autophagy. DAP1 is expressed by most cells and up regulated in many cancer cells as well.
~15 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Immunogen
KLH-conjugated linear peptide corresponding to the unknown of human Death-associated protein 1.
Application
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Death-associated protein 1 in human prostate cancer and normal testis tissues. (Note: It is recommended for end-users to perform a 1:1,000 dilution for enhanced performance.)
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
This Anti-Death-associated protein 1 Antibody is validated for use in western blotting & IHC for the detection of Death-associated protein 1.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in serum starved and control NIH/3T3 cell lysate.
Western Blotting Analysis: 1 μg/mL of this antibody detected Death-associated protein 1 in serum starved NIH/3T3 cell lysate and demonstrated a loss of signal in control NIH/3T3 cell lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
