Description
General description
Bcl-2-modifying factor (Bmf) is a member of the Bcl-2 family containing one BCL2 homology domain 3. Members of this family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cell′s actin cytoskeleton. Bmf is constitutively expressed in many tissues. There are 3 isoforms of Bmf. Isoform 1 is mainly expressed in B-lymphoid cells while isoform 2 and 3 are mainly expressed in B-CLL and normal B-cells.
~25 kDa observed
Immunogen
KLH-conjugated linear peptide corresponding to the N-terminus of human Bmf.
Application
Detect Bcl-2-modifying factor using this rabbit polyclonal antibody, Anti-Bmf Antibody validated for use in western blotting & IHC.
Immunocytochemistry Analysis: 10 µg/mL from a representative lot detected Bmf in HeLa cells.
Biochem/physiol Actions
This antibody recognizes the N-terminus of Bmf.
Analysis Note
Control
HepG2 and HEK293 cell lysates
Evaluated by Western Blotting in HepG2 and HEK293 cell lysates.
Western Blotting Analysis: 1 µg/mL of this antibody detected Bmf in 15 µg of HepG2 and HEK293 cell lysates.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
