Description
General description
POLG is a mitochondrial protein that plays a major role in mitochondrial DNA replication and only for mitochondrial DNA. POLG is the catalytic subunit of a multiple protein complex that makes up the holo-enzyme that is the mitochondrial specific DNA polymerase gamma. POLG makes the alpha subunit of the complex. A number of diseases are associated with mutations that affect the function of POLG and the mitochondria including Leigh syndrome, various neurodegeneration diseases such as Parkinson’s and Alzheimer′s, as well as various skeletal muscle and ophthalmoplegia disorders and even male infertility. The polymerase that POLG ultimately comprises is Polymerase gamma (Pol γ) and is the only DNA polymerase that is active in mitochondria and that can replicate mtDNA.
~140 kDa observed. Uncharacterized band(s) may appear in some lysates.
Immunogen
KLH-conjugated linear peptide corresponding to human DNA polymerase subunit gamma-1/POLG near the N-terminus.
Application
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected DNA polymerase subunit gamma-1/POLG in human cerebellum, human cerebral cortex, human tonsil and human thalamus tissue.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated DNA polymerase subunit gamma-1/POLG in 0.5 mg of HeLa cell lysate.
This Anti-DNA polymerase subunit gamma-1 Antibody/POLG is validated for use in Immunohistochemistry and Immunoprecipitation for the detection of DNA polymerase subunit gamma-1/POLG.
Analysis Note
Evaluated by Immunohistochemistry in human tonsil tissue.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected DNA polymerase subunit gamma-1/POLG in human tonsil tissue.
Other Notes
Replaces: MABN1088
