Description
General description
PGAM5 is a mitochondrial specific phosphatase specific for serine/threonine residues. PGAM5 principally dephosphorylates and activates MAP3K5 kinase a critical player in many cancers. PGAM5 also acts as a central mediator for programmed necrosis or necroptosis, induced by TNF, ROS or calcium. In the mitochondria, PGAM5 may also function as a regulator of mitochondrial dynamics and reconstruction. Also PGAM5 functions in the cellular stress response and regulates the removal of damaged mitochondria via mitophagy. PGAM5 itself is regulated at the protein level by the mitochondrial specific rhomboid protease PARL, which cleaves the N-terminal of PGAM5 in response to mitochondrial depolarization.
~32/34 kDa observed.
Immunogen
Epitope: Near N-terminal
KLH-conjugated linear peptide corresponding to human PGAM5 near the N-terminal.
Application
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
This Anti-PGAM5 Antibody is validated for use in Western Blotting for the detection of PGAM5.
Biochem/physiol Actions
This antibody detects the -L(long) isoform and -S(short) isoform of PGAM5.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in HT-29 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected PGAM5 in 10 µg of HT-29 cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
