Description
General description
Mitochondrial fission 1 protein, hFIs1, is an overexpressed mammlian protein that is localized to the outer mitochondrial membrane of various cell types. It is involved in the fragmentation of the mitochondrial network and its perinuclear clustering, resulting in the release of cytochrome c from the mitochondrion, and apopotosis. It is known to mediate peroxisomal fission and also plays a critical role in histone function by demethylating Lys-4 of histone H3.
~17 kDa observed
Immunogen
Epitope: Cytoplasmic topological domain
KLH-conjugated-linear peptide corresponding to the cytoplasmic topological domain of human Mitochondrial fission 1 protein.
Application
Detect Mitochondrial fission 1 protein (Fis1) using this Anti-Mitochondrial fission 1 protein (Fis1) Antibody validated for use in WB.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Biochem/physiol Actions
Other homologies: Rat and Mouse (83% sequence homology).
This antibody recognizes the cytoplasmic topological domain of Mitochondrial fission 1 protein.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Raji cell lysate
Evaluated by Western Blot in Raji cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Mitochondrial fission 1 protein in 10 µg of Raji cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
