Description
General description
Anoctamin-2, also known as Transmembrane protein 16B (TMEM16B) are subunits of Calcium-activated chloride channels (CaCC) which mediate physiological functions such as epithelial secretion, sensory signal transduction, and smooth muscle contraction. TMEM16B is predicted to have eight transmembrane domains with both the amino and carboxy termini in the cytoplasm and is expressed in several tissues including olfactory sensory neurons as well as photoreceptors in mammalian retina. Like TMEM16A, TMEM16B is thought to form at least part of CaCC’s but has different biophysical characteristics such as voltage dependence and unitary conductance.
~128 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Immunogen
Epitope: N-terminus
KLH-conjugated synthetic peptide corresponding to the N-terminus of human TMEM16B.
Application
Anti-TMEM16B Antibody is an antibody against TMEM16B for use in western blotting, IHC & immunofluorescence.
Immunohistochemistry Analysis: 5 μg/mL from a representative lot detected TMEM16B in human brain tissue.
Immunofluorescence Analysis: 20 μg/mL from a representative lot detected TMEM16B in human brain cells.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Biochem/physiol Actions
This antibody will not cross-react with TMEM16A.
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in rat brain tissue.
Western Blotting Analysis: 2 μg/mL of this antibody detected TMEM16B in rat brain tissue.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
