Description
General description
SFTPC, also known as SFTP2 and Pulmonary surfactant-associated protein C, is a complex mixture of phospholipids and proteins secreted from airway epithelial cells (type II cells) in alveoli which reduces the surface tension at the alveolar air liquid interface, providing alveolar stability necessary for normal ventilation. Four distinct proteins isolated from pulmonary surfactant are termed surfactant proteins A, B, C, and D. SP A (28 - 36 kDa) and SP D (43 kDa) are collagenous carbohydrate binding proteins, whereas SP B (8 - 9 kDa) and SP C (4 kDa) are non collagenous hydrophobic proteins. SP C is a fragment of a much larger precursor protein of 21 kDa. The precursor contains an exceedingly hydrophobic region of 34 amino acids that comprises most of the mature SP C, and a unique poly-valine domain.
~20 kDa observed
Immunogen
Epitope: N-terminus
KLH-conjugated linear peptide corresponding to the N-terminus of human SFTPC.
Application
Detect SP-C using this rabbit polyclonal antibody, Anti-SFTPC Antibody validated for use in western blotting & IHC (Paraffin).
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected SFTPC in human lung tissue.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Rat lung tissue lysate
Evaluated by Western Blotting in rat lung tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected SFTPC in 10 µg of rat lung tissue lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
