Description
General description
The transcription factor NFκB (Nuclear Factor κB) is involved in the expression and regulation of a number of important cellular and physiological processes such as growth, development, apoptosis, immune and inflammatory response, and activation of various viral promoters including human immunodeficiency virus long terminal repeats. NFκB represents a group of structurally related and evolutionarily conserved proteins related to the proto-oncogene c-Rel with five members in mammals that include Rel (cRel), RelA (p65), RelB, NFκB1 (p50 and its precursor p105), and NFκB2 (p52 and its precursor p100). NFκB/Rel proteins exist as homo- or heterodimers to form transcriptionally competent or repressive complexes. Although most NFκB dimers are activators of transcription, the p50/50 and p52/52 homodimers can repress the transcription of their target genes. The p50/p65 heterodimer of NFκB is the most abundant in cells.
~65 kDa observed.
Four isoforms produced by alternative splicing have been described: Isoform 1 (p65) at 60.2 kDa, Isoform 2 (p65 delta 2) at 58.8 kDa, Isoform 3 (p65 delta) at 59.1 kDa, and Isoform 4 at 59.9 kDa
Immunogen
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of human NFκB.
Application
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Use Anti-NFκB p65 Antibody, CT (Rabbit Polyclonal Antibody) validated in WB, IP to detect NFκB p65 also known as transcription factor p65.
Western Blot (SNAP i.d.) Analysis: 5 µg/mL from a representative lot detected NFκB on 10 µg of A431 cell lysate.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated NFκB from A431 RIPA lysate.
Biochem/physiol Actions
This antibody recognizes NFκB at the C-terminus.
Physical form
Format: Purified
Protein A purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
A431 cell lysate
Evaluated by Western Blot in A431 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected NFκB in 10 µg of A431 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 06-418
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
