Description
General description
Histone H3.3 (UniProt: P84243) is encoded by the H3FA3 (also known as H3.3A, H3F3, PP781, H3F3B, H3.3B) gene (Gene ID: 3020) in human. Histones are basic nuclear proteins that are responsible for the nucleosome structure of chromatin in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes, which limits DNA accessibility to the cellular machineries, which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H3.3 is expressed throughout the cell cycle independently of DNA synthesis. It is a variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. It is the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. While H3.3 can function much the same as canonical H3 as a core part of the nucleosome, H3.3 is also deposited into transcriptionally active regions to replace displaced nucleosomes throughout the cell cycle. In tumor cells, H3.3K27M and H3.3G34R/V mutations are heterozygously expressed, with one allele of H3F3A being wildtype. K27M and G34R/V mutations are mutually exclusive in tumors and display distinct gene expression profiles and DNA methylation patterns. Almost all tumors bearing G34R/V mutations also exhibit mutations in ATRX/DAXX and display alternate lengthening of telomeres, which is a classical phenotype of cancerous cells. (Ref.: Yuen, BT., and Knoepfler PS (2013). Cancer cell 24(5); 567-74).
~17 kDa observed; 15.33 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
Conjugated linear peptide correspding to 8 amino acids from the N-terminal region of human histone H3.3 with a G34R mutation.
Epitope: unknown
Application
Anti-Histone H3.3 G34R, Cat. No. ABE403, is a highly specific rabbit polyclonal antibody that targets Histone H3.3 mutant G34R and has been tested for use in Immunohistochemistry and Western Blotting.
Research Category
Epigenetics & Nuclear Function
Biochem/physiol Actions
This rabbit polyclonal antibody specifically detects G34R mutated form of human Histone H3.3. It does not detect widl-type Histone 3.3.
Physical form
Affinity Purified
Format: Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in Hek293 cells transfected with Histone 3.3 G34R mutant.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Histone H3.3 G34R in Hek293 cells transfected with Histone 3.3 G34R mutant.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
