Description
General description
Histone demethylase UTX is also known as Lysine-specific demethylase 6A, Ubiquitously-transcribed TPR protein on the X chromosome, and Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein. UTX is a histone demethylase that specifically demethylates trimethylated and dimethylated but not monomethylated histone H3 ′Lys-27′. UTX is involved in regulation of posterior development, and defects can cause Kabuki syndrome type 2 (KABUK2)
~154 kDa observed. Uncharacterized band(s) may appear in some lysates.
Immunogen
GST-tagged recombinant protein corresponding to human UTX.
Application
Detect Histone demethylase UTX using this rabbit polyclonal antibody, Anti-UTX Antibody validated for use in western blotting & ICC.
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected UTX in HeLa, A431, and 3T3 cells.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Biochem/physiol Actions
Other homologies: Canine (99% sequence homology) and Bovine (96% sequence homology).
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HeLa nuclear extract
Evaluated by Western Blotting in HeLa nuclear extract.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected UTX in 10 µg of HeLa nuclear extract.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
