Description
General description
Histone H3 is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H3 lysine 27 methylation (H3K27) is associated with heterochromatin formation and transcriptional repression.
~ 17 kDa
Immunogen
Branch peptide corresponding to human Histone H3 methylated at Lys27.
Epitope: Lys27
Application
Anti-trimethyl Histone H3 (Lys27) Antibody is a rabbit polyclonal antibody for detection of Histone H3 trimethylated on lysine 27( H3K27me3). This purified polyclonal is specificity verified by dot blot (DB), published in peer reviewed journals and validated in WB, ICC, PIA.
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Histone H3 in NIH/3T3 cells.
Peptide Inhibition Assay: This antibody peptide blocked on HeLa cell extracts.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Biochem/physiol Actions
Broad species cross-reactivity expected.
This antibody recognizes Histone H3 methylated at Lys27.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HeLa acid extract and recombinant Histone H3
Evaluated by Western Blot in HeLa acid extract and recombinant Histone H3.
Western Blot Analysis: 0.1 µg/ml of this antibody detected histone H3 in 10 µg of HeLa acid extract .
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
